SynBio 101 Gene Synthesis & Expression
      'Lab in a Box' designed for College Biology, Chemistry and Chem E Class

OBJECTIVES

1. Learn basic concepts of Synthetic Biology.
2. Learn the PCR-process for gene synthesis and assembly.
3. Sub-clone synthesized DNA protein coding region into a bacterial plasmid using homologous recombination.
4. Transform synthesized DNA into E. Coli and observe gene expression.

"My student were fascinated with this experiment! This kit was a very efficient way to introduce synthetic biology"

Professor Chittur, Ph.D. Chem Eng., University of Alabama in Huntsville

Learn the process of synthesizing and assembling a gene fragment for recombinant protein expression in a non-pathogenic bacterial cell. You will be able to assemble DNA from scratch, insert the genetic material into a plasmid vector, and transfer the product into a living organism expressing a colored protein. This kit allows you to have hands-on experience with the basic tools of state-of-the art genetic engineering.

This laboratory kit is the ideal introduction to synthetic biology. It was designed by biotech professors and students to teach the basics of this new and important field.

(Temporarily Not Available)

DNA sequencing technologies have enabled us to understand the relationship between gene sequences and specific functions. The synthesis of genetic material using several individual oligonucleotides and enzymes has enabled the assembly of many core components that have allowed us to build functional organisms.  The field of synthetic biology promises many advances.  This kit provides the student with a complete experiment to synthesize DNA and observe its gene expression in living cells.